DNA

Part:BBa_K2066548

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: 57S (2 Tet binding cassette)

Sequence from Amit, R., Garcia, H. G., Phillips, R. & Fraser, S. E. Building enhancers from the ground up: a synthetic biology approach. Cell146, 105–118 (2011). Binding cassette that is cloned in the spacer region between the enhancer and promoter and holds two TetO (TetR respressor) binding sites. IN this case, the transfer function should theoretically have three discrete states (unrepressed, intermediate, fully repressed).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 24
    Illegal NheI site found at 119
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None